Excellence in Research and Innovation for Humanity

International Science Index

Commenced in January 1999 Frequency: Monthly Edition: International Paper Count: 10

Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering

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  • 10
    Finding Approximate Tandem Repeats with the Burrows-Wheeler Transform
    Approximate tandem repeats in a genomic sequence are two or more contiguous, similar copies of a pattern of nucleotides. They are used in DNA mapping, studying molecular evolution mechanisms, forensic analysis and research in diagnosis of inherited diseases. All their functions are still investigated and not well defined, but increasing biological databases together with tools for identification of these repeats may lead to discovery of their specific role or correlation with particular features. This paper presents a new approach for finding approximate tandem repeats in a given sequence, where the similarity between consecutive repeats is measured using the Hamming distance. It is an enhancement of a method for finding exact tandem repeats in DNA sequences based on the Burrows- Wheeler transform.
    Sweet Corn Water Productivity under Several Deficit Irrigation Regimes Applied during Vegetative Growth Stage using Treated Wastewater as Water Irrigation Source
    Yield and Crop Water Productivity are crucial issues in sustainable agriculture, especially in high-demand resource crops such as sweet corn. This study was conducted to investigate agronomic responses such as plant growth, yield and soil parameters (EC and Nitrate accumulation) to several deficit irrigation treatments (100, 75, 50, 25 and 0% of ETm) applied during vegetative growth stage, rainfed treatment was also tested. The finding of this research indicates that under deficit irrigation during vegetative growth stage applying 75% of ETm lead to increasing of 19.4% in terms of fresh ear yield, 9.4% in terms of dry grain yield, 10.5% in terms of number of ears per plant, 11.5% for the 1000 grains weight and 19% in terms of crop water productivity compared with fully irrigated treatment. While those parameters in addition to root, shoot and plant height has been affected by deficit irrigation during vegetative growth stage when increasing water stress degree more than 50% of ETm.
    The Influence of using Compost Leachate on Soil Reaction
    In the area where the high quality water is not available, unconventional water sources are used to irrigate. Household leachate is one of the sources which are used in dry and semi dry areas in order to water the barer trees and plants. It meets the plants needs and also has some effects on the soil, but at the same time it might cause some problems as well. This study in order to evaluate the effect of using Compost leachate on the density of soil iron in form of a statistical pattern called ''Split Plot'' by using two main treatments, one subsidiary treatment and three repetitions of the pattern in a three month period. The main N treatments include: irrigation using well water as a blank treatments and the main I treatments include: irrigation using leachate and well water concurrently. Some subsidiary treatments were DI (Drop Irrigation) and SDI (Sub Drop Irrigation). Then in the established plots, 36 biannual pine and cypress shrubs were randomly grown. Two months later the treatment begins. The results revealed that there was a significant variation between the main treatment and the instance regarding pH decline in the soil which was related to the amount of leachate injected into the soil. After some time and using leachate the pH level fell, as much as 0.46 and also increased due to the great amounts of leachate. The underneath drop irrigation ends in better results than sub drop irrigation since it keeps the soil texture fixed.
    In vivo Introduced Extracellular Ubiquitin Regulates Intracellular Processes

    Extracellular ubiquitin in vivo effect on regenerative liver cells and liver histoarchitectonics has been studied. Experiments were performed on mature female white rats. Partial hepatectomy was made using the modified method of Higgins and Anderson. Standard histopathological assessment of liver tissue was used. Proliferative activity of hepatocytes was analyzed by colchicine mitotic index and immunohistochemical staining on ki67. We have found that regardless of number of injections and dose of extracellular ubiquitin liver histology has not been changed, so at tissue level no effect was observed. In vivo double injection of ubiquitin significantly decreases the mitotic activity at 32 hour point after partial hepatectomy. Thus, we can conclude that in vivo injected extracellular ubiquitin inhibits proliferative activity of hepatocytes in partially hepatectomyzed rats.

    Application of Whole Genome Amplification Technique for Genotype Analysis of Bovine Embryos
    In recent years, there has been an increasing interest toward the use of bovine genotyped embryos for commercial embryo transfer programs. Biopsy of a few cells in morulla stage is essential for preimplantation genetic diagnosis (PGD). Low amount of DNA have limited performing the several molecular analyses within PGD analyses. Whole genome amplification (WGA) promises to eliminate this problem. We evaluated the possibility and performance of an improved primer extension preamplification (I-PEP) method with a range of starting bovine genomic DNA from 1-8 cells into the WGA reaction. We optimized a short and simple I-PEP (ssI-PEP) procedure (~3h). This optimized WGA method was assessed by 6 loci specific polymerase chain reactions (PCRs), included restriction fragments length polymorphism (RFLP). Optimized WGA procedure possesses enough sensitivity for molecular genetic analyses through the few input cells. This is a new era for generating characterized bovine embryos in preimplantation stage.
    A Comprehensive Study on Phytoextractive Potential of Sri Lankan Mustard (Brassica Juncea (L.) Czern. and Coss) Genotypes
    Heavy metal pollution is an environmental concern. Phytoremediation is a low-cost, environmental-friendly approach to solve this problem. Mustard has the potential in reducing heavy metal contents in soils. Among mustard (Brassica juncea (L.) Czern & Coss) genotypes in Sri Lanka, accessions 7788, 8831 and 5088 give significantly a high yield. Therefore, present study was conducted to quantify the phytoextractive potential among these local mustard accessions and to assess the interaction of heavy metals, Pb, Co, Mn on phytoextraction. A pot experiment was designed with acid washed sand (quartz) and a series of heavy metal solutions of 0, 25, 50, 75 and 100 μg/g. Experiment was carried out with factorial experimental design. Mustard accessions were tolerant to heavy metals and could be successfully used in removal of Pb, Co and Mn and they are capable of accumulating significant quantities of heavy metals in vegetative and reproductive organs. The order of the accumulative potential of Pb, Co and Mn in mustard accessions is, root > shoot >seed.
    Antibacterial Activity of Lactic Acid Bacteria Isolated from Table Olives against Skin Pathogens
    The aim of this study was to assess the effect of LAB isolated from Iranian native olives on the opportunistic skin pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. Lactic Acid Bacteria were isolated from the brine of each sample in the prior of time. The samples were spread on MRS agar for isolation of lactobacillus and for lactococcus. 28 strains of labs were isolated. The labs were centrifuged, the supernatant was strewed and pellet was used to inoculation in wells or at blank disks. 20μl of each pellet was inoculated to blank disks and 40μl of each pellet was inoculated to each well. The result of disk and well diffusion agar against these pathogens were confirmed each other. The size of inhibition zone was different according to the type of bacteria, the method and the concentrations of labs.
    Effect of Nano-Silver on Growth of Saffron in Flooding Stress
    Saffron (Crocus sativus) is cultivated as spices, medicinal and aromatic plant species. At autumn season, heavy rainfall can cause flooding stress and inhibits growth of saffron. Thus this research was conducted to study the effect of silver ion (as an ethylene inhibitor) on growth of saffron under flooding conditions. The corms of saffron were soaked with one concentration of nano silver (0, 40, 80 or 120 ppm) and then planting under flooding stress or non flooding stress conditions. Results showed that number of roots, root length, root fresh and dry weight, leaves fresh and dry weight were reduced by 10 day flooding stress. Soaking saffron corms with 40 or 80 ppm concentration of nano silver rewarded the effect of flooding stress on the root number, by increasing it. Furthermore, 40 ppm of nano silver increased root length in stress. Nano silver 80 ppm in flooding stress, increased leaves dry weight.
    Chewing behavior and Bolus Properties as Affected by Different Rice Types

    The study aimed to investigate the effect of rice types on chewing behaviours (chewing time, number of chews, and portion size) and bolus properties (bolus moisture content, solid loss, and particle size distribution (PSD)) in human subjects. Five cooked rice types including brown rice (BR), white rice (WR), parboiled white rice (PR), high amylose white rice (HR) and waxy white rice (WXR) were chewed by six subjects. The chewing behaviours were recorded and the food boluses were collected during mastication. Rice typeswere found to significantly influence all chewing parameters evaluated. The WXR and BR showed the most pronounced differences compared with other rice types. The initial moisture content of un-chewed WXR was lowest (43.39%) whereas those of other rice types were ranged from 66.86 to 70.33%. The bolus obtained from chewing the WXR contained lowest moisture content (56.43%) whilst its solid loss (22.03%) was not significant different from those of all rice types. In PSD evaluation using Mastersizer S, the diameter of particles measured was ranged between 4 to 3500 μm. The particle size of food bolus from BR, HR, and WXR contained much finer particles than those of WR and PR.

    Study of Sugarcane Bagasse Pretreatment with Sulfuric Acid as a Step of Cellulose Obtaining
    To produce sugar and ethanol, sugarcane processing generates several agricultural residues, being straw and bagasse is considered as the main among them. And what to do with this residues has been subject of many studies and experiences in an industry that, in recent years, highlighted by the ability to transform waste into valuable products such as electric power. Cellulose is the main component of these materials. It is the most common organic polymer and represents about 1.5 x 1012 tons of total production of biomass per year and is considered an almost inexhaustible source of raw material. Pretreatment with mineral acids is one of the most widely used as stage of cellulose extraction from lignocellulosic materials for solubilizing most of the hemicellulose content. This study had as goal to find the best reaction time of sugarcane bagasse pretreatment with sulfuric acid in order to minimize the losses of cellulose concomitantly with the highest possible removal of hemicellulose and lignin. It was found that the best time for this reaction was 40 minutes, in which it was reached a loss of hemicelluloses around 70% and lignin and cellulose, around 15%. Over this time, it was verified that the cellulose loss increased and there was no loss of lignin and hemicellulose.